Ti-12Nb-12Zr-2Mo alloy has been prepared by water jacketed copper crucible induction melting furnace. The heat treatment was processed using a vacuum furnace. Microstructure and mechanical properties of as-cast and heat treated alloy were systematic invested using XRD, SEM, TEM and mechanical test. The results show that microstructure of as-cast and annealed alloy were composed of α and β phase, while for quenched alloy was α′ and β phase. Heat treatment is conductive to improve strength and remain elastic modulus the same.