TNZS, TiO2/TNZS and HA/TNZS mixed powders were prepared by high-energy milling for 48 h and then sintered by cold pressing. microstructure and phase of TNZS mixed powders and microstructure, phase, pore characteristics and elastic modulus of TNZS-based biomaterials were investigated. The results show that: the three mixed powders are mechanically alloyed after high-energy milling for 48 h; sintering TNZS generates α-Ti phase and β-Ti phase, sintering TiO2/TNZS produces α-Ti phase, β-Ti phase, rutile TiO2 and anatase TiO2; besides of α-Ti, β-Ti and HA, HA/TNZS bio-titanium-based material forms new phases of Ti2O, CaTiO3, CaO and TixPy; the surface composition of the three materials are uniform; porosity of three materials are 2.466%, 5.030% and 13.027%, respectively; elastic modulus of three materials are 64.00 GPa、103.93 GPa and 119.43 GPa, respectively.