Ti-6321 titanium alloy billets have been heated in (α β) phase region and forged at the deformation range of 50%~80% and undergone conventional annealing, we can find that the microtructure types of original billets have not been changed, but the elongated α phase structure have been crushed obviously.With the increasing of deformation, the degree of grain refinement is improved gradually, the strength of bars increased and the impact toughness of bars decreased. The bars with 45 mm diameter have been heat treated by conventional annealing, dual annealing, β annealing, and solution annealing and aging, the result shows that the microstructure and properties of Ti-6321 alloy bars are sensitive to heat treatment process and the optimum heat treatment method should be chosen to achieve a appropriate combination of strength, plasticity and toughness according to the special requirements.