Herein we report an investigation of the piezomagnetic properties and the negative piezomagnetic effect in a Fe73.5Cu1Nb3Si13.5B9 amorphous alloy film. The results show that in its quenched state, the Fe73.5Cu1Nb3Si13.5B9 amorphous alloy film has a significant positive piezomagnetic property. Furthermore, the inductance of the alloy film decreases substantially in the 0–0.66 kPa pressure zone, whereas the compressive stress, σ, increases. At a σ value of 0.66 kPa, the piezomagnetic effect value, SI, reaches 5.5%; The ambient temperature has significant influence on the sensitivity of the piezomagnetic property. The films show optimal piezomagnetic properties and sensitivity stability in the temperature range of 20–30 °C. Increases in ambient temperature lead to increases in the piezomagnetic property and sensitivity values, and the inner stress state of the film changed after annealing treatment. At an annealing temperature of 350 °C, the piezomagnetic effect of the film changes from being "positive " to "negative." Furthermore, as the internal stress decreases, the value of |SI| decreases, and the piezomagnetic property of the film becomes less sensitive to changes in the ambient temperature. For an annealing temperature of 555 °C, the value of |SI| is minimum (0.59%), at a σ value of 0.44 kPa. When the annealing temperature reaches the crystallization temperature, the piezomagnetic property of the film becomes less sensitive to changes in the ambient temperature.