In this paper, Ti-Al-Nb alloy is taken as the research object, the forged Ti-Al-Nb alloy rod is rolled with different deformation and the same heat treatment, and the effects of rolling deformation on the microstructure, mechanical properties at room temperature and 650℃ are studied. The results show that the microstructure of the initial forged samples is dominated by the O-phase plate-like structure, showing the performance matching of high strength and low plasticity. After solution + aging treatment, the microstructure of rolled bar is equal to α2 phase + acicular O phase. When the rolling deformation is 60%, the microstructure of alloy bars is not uniform. With the increase of deformation, the microstructure is uniform, the grain is refined and the plasticity is improved. The amount of rolling deformation has no significant effect on the high temperature properties of the alloy.